The Ku heterodimer, comprised of Ku70 and Ku80 subunits, is a conserved complex involved in nonhomologous end-joining (NHEJ). However, it also functions in maintenance of telomeres, chromosome

Its function remains elusive. We reisolated CLUyXIP8 by yeast two-hybrid analyses using as bait the DNA double-strand break repair protein Ku70. We show that a delayed (2–3 days), low-dose (0.02–10 Gy) IR-inducible nuclear CLUyXIP8 protein coimmunoprecipitated and colocalized (by confocal microscopy) in vivo with Ku70yKu80, a DNA

1997-11-01 · Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. S Jin Division of Tumor Immunology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA. might function as part of a telomeric length sensing system protecting chromosomal termini from nucleolytic attack, as was shown in yeast (Boulton and Jackson, 1996). Recent gene knockout experiments in mice pointed out yet another function of Ku. As expected, both Ku70 and Ku80 knockout mice have First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other. Second, divergent genetic backgrounds or environments inﬂuence the phenotype. To distinguish between these possi-bilities, the Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double Ku70 forms a heterodimer with Ku80, called Ku that is well known for repairing DNA double-strand breaks through non-homologous end joining.

Ku70 and ku80 function

To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine em-bryonic stem cell line which lacks Ku70 expression. We find Ku70 is one component of a protein complex, Ku70 and Ku80, that functions as a heterodimer to bind DNA double-strand breaks and activates DNA-dependent protein kinase. Our previous study with Ku70−/− and Ku80−/− mice, and cell lines has shown that Ku70- and Ku80-deficiency compromises the ability of cells to repair DNA double-strand breaks, increases radiosensitivity of cells, and Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase.

Immunofluorescence.Kinetochore.Ku70.Ku80. Microtubules.Orthoptera.RNAi.Spindleassembly checkpoint Abbreviations Ku70 and Ku80.

Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase.

Required for mating-type switching (By similarity). 2011-01-12 The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double-strand break repair pathway in mammalian cells. Interaction of Ku with DNA is central for the functions of Ku. 2001-03-01 Ku70‐knockout mice, in contrast, appear to be tumor prone and develop thymic lymphomas with high incidence (30, 36).

Its function remains elusive. We reisolated CLUyXIP8 by yeast two-hybrid analyses using as bait the DNA double-strand break repair protein Ku70. We show that a delayed (2–3 days), low-dose (0.02–10 Gy) IR-inducible nuclear CLUyXIP8 protein coimmunoprecipitated and colocalized (by confocal microscopy) in vivo with Ku70yKu80, a DNA

Ku70 and Ku80 (also called Ku86) are encoded by the XRCC6 and XRCC5 genes, respectively, in humans, and have a strong afﬁ nity for free ends of DNA ( 21 ). DNA-PKcs is a 469-kDa protein composed of several distinct functional domains XRCC4, is a protein of unknown function. The Ku70 protein is an additional component of DNA-PK; Ku70 forms a het-erodimer with Ku80 to generate the DNA end-binding com-ponent of the enzyme. To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine em-bryonic stem cell line which lacks Ku70 expression. We find Its function remains elusive. We reisolated CLUyXIP8 by yeast two-hybrid analyses using as bait the DNA double-strand break repair protein Ku70. We show that a delayed (2–3 days), low-dose (0.02–10 Gy) IR-inducible nuclear CLUyXIP8 protein coimmunoprecipitated and colocalized (by confocal microscopy) in vivo with Ku70yKu80, a DNA The propensity for Ku70 to associate with Ku80 and to bind DNA correlates with the ability to activate DNA-PK, although two mutants showed that the roles of Ku70 in DNA-PK activation and IR repair The Ku70/80 heterodimer binds to DNA ends and attracts other proteins involved in the non-homologous end-joining (NHEJ) pathway of DNA double-strand break repair.

We find that the Ku70 -/- cells produce no detectable Ku70 and very little Ku80, suggesting a direct interrelationship between their levels. Both Ku70 and Ku80 therefore contain monopartite NLSs, and sequences outside the basic cluster make favorable interactions with Impα, suggesting that this may be a general feature in monopartite NLSs. We show that the Ku70 NLS has a higher affinity for Impα than the Ku80 NLS, consistent with more extensive interactions in its N-terminal region. function of Ku70 at DSB sites might play a crucial role in modulating the NHEJ repair pathway [3, 14, 20, 26, 28]. There is, however, no report describing the localization and function of canine Ku70.
Peripherin antibody

Find this and comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end binding factor in the ''classical'' non- homologous 26 Dec 2018 The Ku heterodimer, composed of Ku70 and Ku80, is best characterized for its role in repairing double-stranded DNA breaks but is also known Furthermore, it has been reported that the Ku70 subunit contains the ATPase activity and is able to perform helicase function independently of Ku80 (Ochem et 25 May 2007 Summary: Subunit of the Ku70:Ku80 complex; binds RNA and damaged Composed in yeast of Yku70p and Yku80p, Ku functions in genome 17 Jul 2019 We also discuss its lesser-known functions, the pharmacotherapies Ku70 and Ku80 contain three domains: an alpha helix/beta barrel von av K Söderlund Leifler · 2009 — associated with cancer due to gain-of-function mutations are called proto- oncogenes strand break by the end-binding heterodimer of the Ku70 and Ku80 pro-. av P Håkansson · 2006 — regarding the function of an alternative mammalian RNR small subunit, and on the role of is the binding of the end-binding Ku70/Ku80 complex, leading to the. Pia C. Maly Sundgren, 2017 Sep 12, Neuroimaging: Anatomy Meets Function. Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal av P Umate · 2011 · Citerat av 90 — The role of these motifs in ATP-binding, ATP-hydrolysis (ATPase), RNA A total of 31 DNA helicases (like RecQ members, KU70, MCM Protection of Kidney Function with Human Antioxidation Protein Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in 22 jan.

Therefore, Ku70's role in DNA repair may be linked to the known biochemical features of Ku, InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites.
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Function. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system .

In Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal . These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other.